| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cook, Amelia A (8)
|
NIHAMB |
49.89 |
44.36 |
-5.53 |
| 2 |
Hirsch, Cora N (8)
|
NIAAAC |
48.15 |
45.00 |
-3.15 |
| 3 |
Slevinski, Cameryn N (8)
|
NIEMAC |
|
46.77 |
- |
| 4 |
Clouser, Kerrington D (8)
|
NIBCSR |
46.33 |
46.93 |
0.60 |
| 5 |
Yandricha, Elizabeth R (7)
|
NITTAC |
1:10.00 |
47.03 |
-22.97 |
| 6 |
Baker, Catherine M (7)
|
NITWST |
45.47 |
47.86 |
2.39 |
| 7 |
Rydzewski, Alivia A (8)
|
NITWST |
1:05.44 |
49.64 |
-15.80 |
| 8 |
Ellis, Kuepper M (8)
|
NIPVSC |
56.06 |
51.17 |
-4.89 |
| 9 |
Girard, Sydney V (8)
|
NIEAST |
|
52.09 |
- |
| 10 |
Ferullo, Hannah G (8)
|
NITWST |
52.65 |
53.95 |
1.30 |
| 11 |
Loehfelm, Madelyn G (6)
|
NITWST |
52.26 |
54.83 |
2.57 |
| 12 |
Wagner, Addison (7)
|
NITWST |
|
59.31 |
- |
| 13 |
Cone, Sadie L (8)
|
NITTSC |
58.36 |
1:03.46 |
5.10 |
| 14 |
Marcyan, Reese J (6)
|
NITTSC |
1:00.59 |
1:03.97 |
3.38 |
| 15 |
Seelig, Jordan L (8)
|
NIEAST |
|
1:04.06 |
- |
| 16 |
Hosmer, Sadie M (8)
|
NIPVSC |
|
1:08.00 |
- |
| 17 |
Marusza, Ava J (7)
|
NITWST |
1:17.67 |
1:09.88 |
-7.79 |
| 18 |
Evans, Gwenyth C (8)
|
NIHAMB |
1:19.88 |
1:10.93 |
-8.95 |
| 19 |
Hufnagel, Megan K (5)
|
NITTSC |
|
1:12.63 |
- |
| 20 |
Sellan, Grace I (7)
|
NITTAC |
1:20.00 |
1:29.68 |
9.68 |
| 21 |
Chmielowiec, Basia K (6)
|
NIEAST |
|
1:32.09 |
- |
|
Hellwig, Allison R (8)
|
NIHHSC |
|
DQ |
- |